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pcdna3 1 ha ubiquitin  (MedChemExpress)


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    Structured Review

    MedChemExpress pcdna3 1 ha ubiquitin
    Pcdna3 1 Ha Ubiquitin, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 2204 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna3+1+ha+ubiquitin/MG-132/pmc12658001-200-19-37
    Average 99 stars, based on 2204 article reviews
    pcdna3 1 ha ubiquitin - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Immunoprecipitation:

    Article Title: Highly efficient and long-acting split-and-mix proteolysis targeting chimera based on self-assembled polylactic acid
    Article Snippet: Primary antibodies included BRD4 (1:1000; Abcam, ab128874), ERα (1:1000; CST, #8644), CDK4 (1:1000; CST, #12790), β-Tubulin (1:1000; CST, #15115) and GAPDH (1:5000; CST, #97166). .. For immunoprecipitation, cells were seeded into 6-well cell culture plates for 24 h. Then the cells were transfected with pcDNA3.1-HA-ubiquitin for 48 h and treated with the degradation group (with or without 10 μM MG132 or MLN7243 (MedChemExpress, HY-13259) or 50 μM Pomalidomide) for 8 h or 24 h. Subsequently, total cell protein was obtained and determined according to the steps above. .. Finally, the protein lysate was preincubated with anti-HA rabbit antibody (1 μg) at 4 °C overnight, and 30 μL Protein G Sepharose (Cytiva, Shanghai, China) was added for 2 h at 4 °C.

    Cell Culture:

    Article Title: Highly efficient and long-acting split-and-mix proteolysis targeting chimera based on self-assembled polylactic acid
    Article Snippet: Primary antibodies included BRD4 (1:1000; Abcam, ab128874), ERα (1:1000; CST, #8644), CDK4 (1:1000; CST, #12790), β-Tubulin (1:1000; CST, #15115) and GAPDH (1:5000; CST, #97166). .. For immunoprecipitation, cells were seeded into 6-well cell culture plates for 24 h. Then the cells were transfected with pcDNA3.1-HA-ubiquitin for 48 h and treated with the degradation group (with or without 10 μM MG132 or MLN7243 (MedChemExpress, HY-13259) or 50 μM Pomalidomide) for 8 h or 24 h. Subsequently, total cell protein was obtained and determined according to the steps above. .. Finally, the protein lysate was preincubated with anti-HA rabbit antibody (1 μg) at 4 °C overnight, and 30 μL Protein G Sepharose (Cytiva, Shanghai, China) was added for 2 h at 4 °C.

    Transfection:

    Article Title: Highly efficient and long-acting split-and-mix proteolysis targeting chimera based on self-assembled polylactic acid
    Article Snippet: Primary antibodies included BRD4 (1:1000; Abcam, ab128874), ERα (1:1000; CST, #8644), CDK4 (1:1000; CST, #12790), β-Tubulin (1:1000; CST, #15115) and GAPDH (1:5000; CST, #97166). .. For immunoprecipitation, cells were seeded into 6-well cell culture plates for 24 h. Then the cells were transfected with pcDNA3.1-HA-ubiquitin for 48 h and treated with the degradation group (with or without 10 μM MG132 or MLN7243 (MedChemExpress, HY-13259) or 50 μM Pomalidomide) for 8 h or 24 h. Subsequently, total cell protein was obtained and determined according to the steps above. .. Finally, the protein lysate was preincubated with anti-HA rabbit antibody (1 μg) at 4 °C overnight, and 30 μL Protein G Sepharose (Cytiva, Shanghai, China) was added for 2 h at 4 °C.



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